A-Scan tissue oxygen scanner is uniqely designed for reseachers. It combines a rugged design for repeated use in tissue with an easy and intuitive depth scanning.

The sensor is constructed of a robust polymer-coated glass capillary, combining longitudinal rigidity for easy tissue penetration, with high flexibility for off-axis forces. Oxygen level can be measured at any point along the side of the probe. Automatic scanning can be programmed and repeated periodically with NO relative motion between the probe and the tissue. This feature is ideal for pre-clinical studies in animal models. Oxygen gradients are accurately recorded in a form of a full pO2 depth profile. Dynamic response to treatment or other ques can be explored by programming repeated scans in short time intervals.


Technical Specifications (highlights)

 The Blueberry Test

Fruits respiration, the process of consuming oxygen for ATP production, continue even after harvesting, packaging, and transport to your local grocery store. The limited rate of oxygen diffusion through the outer shell of the fruit results in a low pO2 level in the inner parts of the fruit.

Here in A-Scan's lab we love blueberries. They are perfect for testing. Since their skin is pretty thin, we can estimate the spatial resolution of the system by scanning a blueberry. The figure below shows a resolution of better than 0.4 mm.

We can also use them to estimate the response time of the sensor. Starting a pO2 measurement at a fixed point inside the blueberry as a function of time and then abruptly removing the blueberry from the probe is our equivalent of a step response test. The results show a response time of approximately 5 s.

Blueberry in a water tank. 

Needle probe is inserted through the blueberry

Spatial distribution of dissolved oxygen in the blueberry

Temporal response at a fixed position (14mm) to abrupt removal of the blueberry

An Application Example

A scan through a cancer tumor in a mouse cancer model demonstrating a sharp decrease of pO2 in the tumor core. For more details see S. Ashkenazi et. al.

Ashkenazi S., Cho D., Song C.W. (2021) Scanning Tissue Oxygen Needle Probe. In: Nemoto E.M., Harrison E.M., Pias S.C., Bragin D.E., Harrison D.K., LaManna J.C. (eds) Oxygen Transport to Tissue XLII. Advances in Experimental Medicine and Biology, vol 1269. Springer, Cham. https://doi.org/10.1007/978-3-030-48238-1_8

Probe positioning in a tumor in a hindlimb of a mouse

Tissue oxygen scan through a cancer tumor. Hypoxic core is observed

A-Scan System and Probe

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